ABSTRACT

Objective:

It was investigated whether patients with neurofibromatosis type 1 (NF1) with and without optic glioma (OG) differed from the healthy control group in terms of diffusion tensor imaging (DTI) parameters obtained from optic radiation.

Methods:

Eighty three patients and 36 healthy controls followed with the diagnosis of NF1 were included in the study. Routine MRI and DTI were applied to all subjects. Fractional anisotropy (FA), mean diffusity (MD), radial diffusity (RD) and axial diffusity (AD) values were calculated by placing ROI on the right and left optic radiation on colored FA maps. Patients with OG detected by conventional magnetic resonance imaging (n=19) were classified as group 1, patients with NF1 without OG (n=64) were classified as group 2 and they were compared with the healthy control group in terms of DTI parameters.

Results:

The right and left optic radiation FA values of the patients in group 1 and group 2 were significantly different when compared with the healthy control group. Optical radiation FA values were significantly lower than healthy control group. MD values in group 1 patients with OG were significantly higher than healthy control group. Optic radiation RD values were significantly higher in group 1 and group 2 compared with healthy control group.

Conclusion:

There appears to be microstructural damage in optic radiation in patients with NF1 with or without OG. The idea that changes in FA values detected in optic radiation in visual pathways in patients with NF1 can be predicted in the early period of visual impairment is promising in terms of treatment planning and management.

Keywords:

Neurofibromatosis type 1, optic gliom, diffusion tensor imaging, fractional anisotropy

Introduction

Neurofibromatosis type 1 (NF1) is an autosomal dominant disease that results in decreased production of neurofibromin, a tumor suppressor protein, due to mutation or deletion in the NF1 gene on chromosome 17. NF1 affects almost all organs and systems in the body. Pigment abnormalities (café-au-lait macules, skinfold frecklings, Lisch nodules), peripheral and central nervous system tumors (neurofibromas and gliomas), learning and attention problems, autism spectrum disorders, bone abnormalities (long bone dysplasias, scoliosis), seizures, sleep disorders, and vasculopathies (moyamoya syndrome, renal artery stenosis) are common in affected patients (1,2).

The incidence of optic glioma (OG) in children with NF1 is approximately 15-20%, and vision loss accompanies up to 35-50% (1-4). OGs are usually seen in children younger than 7 years old. Determining the timing of treatment of NF1-associated OGs appears to be a clinical challenge. Visual symptoms include loss of visual acuity, proptosis, strabismus, and nystagmus. NF1-related OGs are infiltrative and tend to progress along the optic nerves, optic chiasm, optic tract, and optic radiations (5-7). OGs that develop in the optic pathway after the optic chiasm tend to exhibit more aggressive clinical behavior than those involving the optic nerve or chiasm. In patients in whom the optic tract and radiation are affected, visual acuity deficiency is mostly seen. Surgical resection is not very suitable because of the irregular tumor margins of OGs and the risk of vision loss.

A reliable criterion predicting future vision loss in patients requiring treatment has not been defined yet (1-3,8). It is not possible to predict the development of vision loss in patients with NF1 with conventional MRI. There is no relationship between tumor size on MRI and visual impairment (2,7). Diffusion tensor imaging (DTI), an advanced neuroimaging method, shows the relationship between white matter structures and neural functions. Both mean diffusity (MD) and fractional anisotropy (FA) values provide information about microstructural changes in white matter. There are studies advocating that future visual acuity loss can be predicted in NF1-related OG with DTI (2).

In our study, we aimed to investigate whether patients with NF1 with and without OG differed from the healthy control group in terms of DTI parameters in optical radiation of the visual pathways.

Method

Eighty three patients (43 men and 40 women, mean age; 10.51±5.63) followed with the diagnosis of NF1 and 36 age-matched healthy controls (18 men and 18 women, mean age; 10.69±5.48) were included in the study and evaluated retrospectively. Routine MRI and DTI were applied to all patients with NF1 and healthy control group. The following parameters were used in the imaging protocol; axial and sagittal T2 images (TR/TE: 4.280/91ms matrix: 384×211; NSA: 1; slice thickness 5 mm), axial T1 images (TR/TE: 500/87 ms; matrix: 256×125; NSA: 1; slice thickness 5 mm), axial and coronal FLAIR images (TR/TE/TI: 8,000/118/23.687 ms; NSA: 1; slice thickness 5 mm), axial and coronal T1 images with contrast (TR/TR: 448/ 87; matrix: 256x134; NSA: 1), and 3DT1 postcontrast sagittal images (TR/TE: 476/86; matrix: 256x154; NSA: 1; slice thickness 1mm). The protocol determined by obtaining DTIs in the axial plane was applied (TR=6,000 ms, TE=89 ms, 30 directions, b=1,000 s/mm², 5-mm section thickness, 230 mm FOV and matrix: 128x128). FA, MD, RD (radial diffusity) and AD (axial diffusity) values were calculated by placing ROI on right and left optical radiation on colored FA maps on a Siemens Leonardo workstation (Figure 1). Patients with NF1 with OG detected by conventional MRI (n=19) constituted group 1, those with NF1 without OG (n=64) constituted group 2; and healthy control group (n=36) were compared in terms of DTI parameters (Figure 2a, b).

Statistical Analysis

All statistical analyzes were performed with IBM SPSS 19.0. The normal distribution of the groups was evaluated with the Kolmogirov-Smirnov test. Comparisons between patients with NF1 with and without OG and healthy controls were analyzed using the Mann-Whitney U test. A p value less than 0.05 was considered statistically significant.

Results

Optical radiation FA, MD, AD and RD values in patients with NF1 with and without OG and in healthy control group are presented in Table 1.

When group 1 and healthy control group were compared;

1- When the right and left optical radiation FA values were compared with the healthy control group, a significant difference was found (p=0.0001 for each). Optic ratio FA values were significantly lower in patients with NF1 with OG compared to healthy controls.

2- Right and left optical radiation MD values were significantly higher than the control group (p=0.02 and p=0.01, respectively).

3- When the optical radiation AD values were compared with the healthy control group, no significant difference was found.

4- Right and left optical radiation RD values were significantly higher than the healthy control group (respectively; p=0.01, p=0.0001).

When group 2 and healthy control group were compared;

1- When the FA values of the right and left optic radiation were compared with the healthy control group, there was a significant difference (p=0.0001 for each). Optic ratio FA values were significantly lower in patients with NF1 without OG compared to healthy controls.

2- When the right and left optical radiation MD and AD values were compared with the control group, no significant difference was found.

3- Right and left optical radiation RD values were significantly higher than the healthy control group (respectively; p=0.001, p=0.002).

When group 1 and group 2 were compared;

1- No significant difference was found in terms of optical radiation FA values.

2- Right and left optical radiation MD values were higher in group 1 (p=0.02 and p=0.05, respectively).

3- No significant difference was found in terms of AD values.

4- Left RD values were significantly higher in Group 1 (p=0.003).

Discussion

Although OGs have a histologically benign nature, low proliferative potential and excellent survival results, they can cause serious problems that may result in irreversible blindness (5). It may affect the optic nerve or prechiasmatic area unilaterally or bilaterally in approximately one third of children with NF1 under the age of seven (6,9,10). Symptoms may not be evident in the early stages of the disease. Findings such as obstructive hydrocephalus, sudden unexplained visual loss, nystagmus, visual field loss, and diencephalic syndrome may not always be observed in children with OG. The diagnosis is made in the early period by clinical and radiological findings (5,6).

It is thought that the visual impairment observed in OG may be related to perineural or intraneural tumor growth and damage in the nerve’s inner structure. Perineural growth occurs as a result of proliferation of astrocytes with some neural intra-tumor components without cystic changes, and fibrovascular arachnoidal trabeculae, mucinous and microcystic degeneration. Intraneural growth represents interaxial astrocytic proliferation and enlargement of fibrovascular trabeculae (5).

Management of OG is possible with careful evaluation of tumor-related parameters, including histological subtype, tumor location and extension, age, and presence of predisposing conditions, especially NF1 (5). Among the treatment options are observation, surgery and chemotherapy aimed at controlling tumor growth. Timing of treatment depends on tumor location and clinical features of other critical functional areas, visual disturbances due to OG, and treatment-related visual risks (5,10-12). Clinical follow-up and treatment decisions in children with NF1-related OG are made by MRI follow-up and ophthalmological examination (8). Vision loss in OGs can probably be explained by direct infiltration or pressure on the visual pathways by the tumor (8).

Although conventional MRI findings do not correlate with tumor size and visual loss, direct measurements of the integrity of the white matter tracts in the optic tract can better show visual loss. DTI can show the identification of all white matter tracts, including optic nerves, optic chiasm, optic tracts, and optic radiations, and whether they are affected at the cellular level (8).

DTI is an advanced imaging method that noninvasively evaluates cerebral white matter integrity and microstructural changes. Tissue anisotropy provides important information about axonal packaging, water-dependent membrane permeability, internal axon structure, degree of myelination, and general tissue water content (13,14). The degree of tissue anisotropy is measured by FA. Axial diffusivity (AD, λ1) measures the diffusion of water molecules parallel to the main axis of the axons and radial diffusivity (RD) (mean of λ2 and λ3) measures the diffusion of water molecules perpendicular to the main axis of the fibers. Increased AD indicates axonal damage, and increased RD is thought to be associated with myelin deficiency or damage (28,29). Myelin loss and axonal damage occur as a result of a long-term disease process. Increased MD values in hamartomatous lesions in the brain seen in NF1 have been associated with histopathologically reported increased water accumulation, myelin vacuolation and spongiotic changes. Decreased FA and increased AD and RD values occur as a result of myelin and axonal damage (13,14).

Evaluation of visual pathways using DTI is important in terms of analyzing the potential risk of visual loss (15). In recent years, it is thought that DTI parameters can be used as a biomarker in the evaluation of pathologies affecting the visual pathways such as optical radiation (15). Optical radiations transmit visual information from the lateral geniculate nucleus to the primary visual cortex. FA in the visual pathways have been associated with visual acuity or disorders such as optic neuritis, visual pathway tumors, prematurity, and optic neuropathy.

There are opinions that FA values can be used as a radiological biomarker in the evaluation of visual acuity in children with OG and can guide treatment (15). In a study, it was reported that there was a correlation between FA values and visual acuity loss in optical radiations of patients with NF1-related OG (8). Optical radiation FA values in subjects with abnormal visual acuity but no tumor involvement were significantly lower than those with normal visual acuity (8). Low FA values in optical radiations were associated with decreased visual acuity. In that study, it was emphasized that microstructural white matter integrity was more important than the number and density of fibers in the visual pathways (8). While white matter tract integrity in optic radiations was associated with loss of visual acuity, no changes in white matter integrity were observed in optic nerves and optic tract. Changes in optic radiation were reported in patients with OG in the anterior visual pathways (8,16,17). In another study, decreased FA values in optic nerve and optic radiation were reported in patients with NF1 compared to healthy controls (8,16). Lober et al. (17), on the other hand, found a decrease in the number of visual tracts in their study with a small number of patients, but they did not find a relationship between this decrease and visual acuity (18). In our study, optical radiation FA values in patients with NF1 with OG showed a significant decrease compared to the healthy control group, while MD and RD values were higher. Decreased FA and increased MD and RD values in the visual pathways in patients with NF1 were thought to be associated with the impairment of optic radiation at the microstructural level and myelin loss and demyelination. In addition, the increase in MD values in optic radiation in patients with NF1 with OG compared to healthy controls may be related to myelin vacuolation and spongiotic changes due to increased water accumulation reported in the histopathology of hamartomatous lesions. In patients with NF1 without OG, decreased FA and increased RD values were detected in optical radiation. These findings support the hypothesis that NF1 may be associated with microstructural damage in visual pathways independent of the presence of OG. There was no difference between the patients with NF1 with and without OG in terms of optical radiation FA values. This supports the hypothesis that changes similar to the myelin disorder reported in hamartomatous lesions seen in NF1 also develop in the visual pathways.

Study Limitations

Among the limitations of our study, first of all, small white matter structures of visual pathways such as optic nerves, optic chiasm, and optic tract were difficult to isolate on DTI maps due to the partial volume effect, and these regions were excluded from the study because these pathways were exposed to sensitivity artifacts. Our second limitation was the small number of patients with NF1 with OG. Our third limitation was that examination findings such as visual field and visual acuity in patients with NF1 were not included in our study.

Conclusion

As a result, it is obvious that there is microstructural damage in optic radiation, which is one of the visual pathways, in patients with NF1 with or without OG. Optical radiation FA values are thought to be related to the decrease in visual acuity during the follow-up period (8). Despite the variability in DTI parameters, extensive prospective studies are needed to determine the FA threshold value associated with visual loss and decreased visual acuity. The idea that changes in FA values detected in optical radiation in the visual pathways in patients with NF1 can be predicted in the early stages of visual impairment is promising in terms of treatment planning and management.

Ethics

Ethics Committee Approval: 2020-10288.

Peer-review: Externally peer reviewed.

Financial Disclosure: The author declared that this study received no financial support.

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Evaluation of Optical Radiation from Visual Pathways with Diffusion Tensor Imaging in Patients with Neurofibromatosis Type 1